Tfnr antibody
Web16 Jan 2015 · TfnR staining was performed using anti-TfnR antibody (H68.4) unless indicated otherwise. For the stain of endogenous EHD1, cells were fixed with pre-warmed 2% para-formaldehyde in PBS at room temperature for 10 min, quenched with 50 mM NH 4 Cl, and permeabilized with 0.1% Triton X-100 in PBS at room temperature for 5 min. After … WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Tfnr antibody
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Web4 Nov 2011 · Sources of antibodies used in the present study were as follows: polyclonal rabbit anti-TGN46 ( 28 ), a kind gift from Minoru Fukuda, Burnham Institute; monoclonal mouse anti-transferrin receptor (TfnR) (H68.4), Zymed Laboratories Inc.; monoclonal mouse anti-GM130, anti-EEA1, and anti-Lamp-1, BD Biosciences; monoclonal rat anti-HA (3F10), … Web8 Apr 2024 · To confirm this interpretation, we immunoisolated TfnR containing vesicles from cell extracts with anti-TfnR antibody and analyzed them by immunoblotting for the presence of endosomal and ...
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WebFirst primary antibodies (same as for Western blots) were applied at 1:10 dilution in PBS/1% BSA, and a bridging antibody used for the polyclonal anti-rab4 antibody. 5-nm gold labeled secondary antibody was then applied (H. Geuze). The grids were washed and further fixed with glutaraldehyde. Web16 Nov 2012 · ( C) HSG cells and intact SGs were labeled with antibodies against VAMP8 (red) and TfnR (green). HSG cells were also labeled with Hoechst (blue), whereas SGs were labeled with phalloidin (Cyan). Note that in SGs VAMP8 is localized close to the apical plasma membrane and does not colocalize with TfnR (lower panel). Bars, 10 μm. Figure 2.
Web11 Nov 2007 · At this time, the levels of SNX4, TfnR, KIBRA and the loading control β-actin were determined by western blotting with specific antibodies. Full blots are shown from cropped transfer membranes.
Web15 Jul 2015 · Polyclonal rabbit anti-SNAP23 antibody and monoclonal mouse anti-TfnR antibody (CD71) were from Sigma-Aldrich. Monoclonal mouse antibodies against β-tubulin (KMX-1) and actin (C4) were from Millipore. Monoclonal mouse anti-Sec8 (clone 14) and … cow picture framecow picture coloring pageWeb13 Feb 2006 · Tfn bound to the TfnR is believed to move sequentially through Rab4-positive EEs and, at least in part, through REs that contain Rab11 before being returned to the cell surface and released. To try to quantify initial rates of Tfn uptake, we measured Tfn content of cells in the first 7 min of uptake ( Fig. 5 A ). cow picture for kitchenWebHern á ndez et al. observed a reasonable safety profile for TNF-α antibodies and argue that the benefits far outweigh the possible risk of adverse events [142].There are several studies and meta-analysis dealing with these TNF-α antibodies and which are extensively reviewed elsewhere. Interleukin-33 is the newest member of the IL-1 family and located on … disneyland california galaxy\u0027s edgeWebCurtis Okamoto, ...Xuebiao Yao, in Physiology of the Gastrointestinal Tract (Fifth Edition), 2012. 46.2.7 SNAREs in Vesicle Docking and Priming. The specificity and driving force for membrane fusion have been fascinating subjects in the cell biology of exocytosis, with a great deal of research centered on supporting or challenging what has become known as … cow pictures clipartWeb12 Apr 2004 · The TfnR is no more susceptible to proteolysis after mVPS26 knock down and the intracellular protein Snx1 is unaffected by addition of the protease, showing that proteolysis was restricted to only cell surface proteins. cow picture for kidsWeb12 May 2003 · Of the three endocytic receptors mentioned above, we focused on LRP because the anti-TfnR mAb, H68, gave a high background in the presence of rat brain cytosol, making it difficult to detect TfnR by Western blotting and the ASGPR was less efficiently recruited into the HSP. disneyland california food