Webto the injection region. The enzymatic hydrolysis of fluorescein diphosphate by alkaline phosphatase demonstrates the ability of this microfluidic FIA device to perform rapid, sensitive assays with limited sample consumption. EXPERIMENTAL SECTION Reagents and Solutions. All solutions were prepared in 18-M¿ deionized water (Millipore, Bedford, … WebFluorescein diphosphate tetraammonium is a fluorescent dye, it can be used as a fluorogenic substrate and generats fluorescein as a fluorescent product. For research use only. We do not sell to patients. Fluorescein …
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WebMar 1, 2008 · Alkaline phosphatase was chosen as a model enzyme and used to convert fluorescein diphosphate into fluorescein. Droplets of alkaline phosphatase and fluorescein diphosphate were merged and mixed on the device, resulting in a 140-nL, stopped-flow reaction chamber in which the fluorescent product was detected by a … WebFeb 1, 1999 · PDF A highly sensitive and continuous protein tyrosine phosphatase (PTPase) assay using 3,6-fluorescein diphosphate (FDP) is described. Leukocyte... … grade 1 reading expectations ontario
64013-70-3;卡那霉素二硫酸盐;51333-22-3;布地奈德; CAS
WebTarget IC 50: 6 nM for dephosphorylation of fluorescein diphosphate by Wip1 (2-240) and 13 nM for phospho-p38 MAP kinase (Thr180) Warning. Toxicity: Standard Handling (A) Reconstitution. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. WebFluorescein diphosphate, tetraammonium salt Description Ultrasensitive fluorogenic alkaline phosphatase substrate Biological description Ultrasensitive fluorogenic alkaline phosphatase substrate. Hydrolysis produces the green fluorescent dye, fluorescein (Ex/Em = 490/520 nm). Purity > 98% CAS Number 217305-49-2 Chemical structure Properties … WebJul 2, 2016 · A highly sensitive and continuous protein tyrosine phosphatase (PTPase) assay using 3,6-fluorescein diphosphate (FDP) is described. Leukocyte phosphatase … chil notebook covers