WebDive into the research topics of 'Identity of the Axial Ligand of the High-Spin Heme in Cytochrome Oxidase: Spectroscopic Characterization of Mutants in the bo-type Oxidase of Escherichia coli and the aa3-type Oxidase of Rhodobacter sphaeroides'. Together they form a unique fingerprint. Oxidase Biochemistry, Genetics and Molecular Biology WebJan 21, 2024 · Improved cyclopropanation activity of histidine-ligated cytochrome P450 enables the enantioselective formal synthesis of levomilnacipran Angewandte Chemie 2014 Cover article. Mutation of the...
Strategic Roles of Axial Histidines in Structure Formation …
WebSep 27, 2024 · Recent work using the engineered Nδ-methyl histidine-ligated cytochrome c peroxidase highlighted changes in spectroscopic and catalytic properties upon axial … WebJan 11, 2001 · The inability of imidazole to complement function in the axial histidine deletion mutant, H175G, of yeast cytochrome c peroxidase has been an intriguing but unresolved issue that impacts our understanding of the role of axial ligands in heme catalysis. Here we report the functional and spectroscopic properties of H175G and of its … philip birnbaum architect
Bis-histidine-coordinated hemes in four-helix bundles: how
WebThe effect of the protein on the ligand binding properties of a ferric porphyrin was investigated by determining spectrophotometrically the temperature dependence of the binding constants, log K_(eq) WebCytochrome c oxidase is a complex, seven subunit enzyme containing two copper atoms, Cu, and CUB, and two hemes, the low-spin heme a and the high- ... heme a3 structure using a deprotonated axial histidine [S] (fig.2a) has been strongly adopted by Palmer [3]. Supportive evidence comes from several hemoglobin- like properties of heme ~3 ... WebIn this study, we attempted to design an artificial peroxidase based on cytochrome c (cyt c), whose heme is covalently bound to the protein. We found that cyt c can degrade RB19, … philip b. k. potter