Alexa phalloidin 488

Author: wjrb

August undefined, 2024

WebAdd Phalloidin-iFluor™ 488 Conjugate solution (100 μL/well) Stain the cells at room temperature for 20 to 90 minutes Wash the cells Examine the specimen under microscope with FITC filter Important Warm the vial to … WebPhalloidin, Alexa Fluor ™ Plus 647 Phalloidin, and Alexa Fluor Plus 750 Phalloidin. Note: For a complete list of fluorescent or biotinylated phalloidin conjugates, see Table 1 on page 5. Contents and storage Contents Cat. No. Amount[1] Storage[2] Fluorescent phalloidin See Table 1 on page 5. 1 vial of lyophilized solids (300 units/assay[3 ...

Phalloidin staining protocol Abcam

WebSix individual channels are captured via staining with Hoechst 33342 (channel 1, blue), Alexa Fluor 488 Concanavalin A (channel 2, green), Alexa Fluor 568 Phalloidin (channel 3, red), Syto14 (channel 4, cyan), MitoTracker Deep Red FM (channel 5, magenta), Alexa Fluor 555 Agglutinin (channel 6, yellow). WebXFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488* Catalog number: 23153 Unit size: 300 Tests Product Details Storage Conditions Freeze (<-15 °C), Minimize light … almira morrone

Alexa Fluor® 488 Phalloidin

WebActi-stain™ 488 phalloidin-labeled actin filaments retain many functional characteristics of unlabeled actin including their ability to interact with myosin. Actin-stain™ 488 phalloidin is supplied as an orange solid. … WebAlexa Fluor ® 488 Phalloidin allows researchers to fluorescently stain the cytoskeleton through the binding of phalloidin to F-actin. This product is intended for use on fixed and … WebAdd XFD488 Phalloidin Conjugate solution (100 μL/well) Stain the cells at room temperature for 20 to 90 minutes Wash the cells Examine the specimen under … almirantazgo significado

Alexa Fluor® 488-Rabbit Anti-His Tag Antibody JIR - Jackson …

The SF3B1R625H mutation promotes prolactinoma tumor …

WebActinGreen 488 reagent is formulated as a room temperature-stable solution of Alexa Fluor 488 Phalloidin (Cat. No. A12379) and comes in a convenient dropper bottle. Just tip and … WebNov 23, 2024 · Phalloidine is a very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of... almirante azarolaWebFlash Phalloidin™ Green 488 excites maximally at 488 nm and emits maximally at 520 nm. Phalloidin is a bicyclic peptide that can be found naturally in the death cap mushroom. This molecule is considered to bind so tightly to F-actin that when ingested by an organism, it will prevent the depolymerization of the actin polymeric filaments which ... almirantazgo fjord

"WebPhalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized. " - Alexa phalloidin 488

Alexa phalloidin 488

Invitrogen™ Alexa Fluor™ 488 Phalloidin - Fisher Sci

WebAlexa Fluor® 488. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing ... WebAlexa Fluor® 488 Phalloidin can be diluted 1:20 (5 μl per 100 l assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once …

Did you know?

WebAlexa Fluor 488® phalloidinis a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Selectively stains F … WebImmunofluorescent analysis of A549 cells using 13573-1-AP (TGN46 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Save time and replace your secondary antibodies with our FlexAble Antibody Labeling Kits $389 / 150 μL Cat No. 13573-1-AP In stock.

WebApr 8, 2024 · F-actin and nuclei were labeled with Alexa Fluor 488 phalloidin (green) and 4’,6-diamidino-2-phenylindole (DAPI, blue). Scale bar, 50 μm. f, g Fluorescence imaging of EGFP expression (f) and percentages of EGFP-positive cells (g) in B16-F10 cells and the other 7 types of cells after NP Tyr-C9AP transfection. Scale bar, 50 μm. WebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes

WebKeyword:'alexa fluor 488 phalloidin' Showing 1-30 of 122 results for "alexa fluor 488 phalloidin" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. Filter & Sort. All Photos (1) WebJul 1, 2024 · Phalloidin-iFluor ® 488 conjugate PBS 3.0 - 4.0% Methanol-free formaldehyde 0.1% Triton X-100 BSA Cell culture or tissue sample for staining Optional: ethanolamine Optional: FluoroQuest Mounting Medium with DAPI Actin Staining Protocol Note: If using paraffin-embedded tissue samples, a deparaffinization and rehydration step is required.

WebAlexa Fluor Hg‐Link dye is a new generation of fluorescent probes that exhibit some advantages over other fluorescent dyes, such as being highly fluorescent over a broad pH range (pH 4 to 10), good water solubility, and higher photostability, allowing more time for …

Web7 rows · Alexa Fluor® 568 Alexa Fluor® 647 ... CoraLite® Plus 488-Phalloidin (green) Ex/Em: 490/515nm. Storage. Store at -20°C. Avoid exposure to light. Stable for 2 years … almirante aniceto rosique nietoWebAlexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. • Invitrogen™ Alexa … almirante barroso google mapsWebPhalloidin is a toxin isolated from mushrooms that binds F-Actin in the cytoskeleton. This product is conjugated to our fluorescent CoraLite (R) dye to stain F-actin for labeling, identifying and quantifying actin filaments in immunostaining studies. almirante cego one pieceWebAdd XFD488 Phalloidin Conjugate solution (100 μL/well) Stain the cells at room temperature for 20 to 90 minutes Wash the cells Examine the specimen under microscope with FITC filter Important Warm the vial to room temperature and centrifuge briefly before opening. Storage and Handling Conditions almirantazgo san petersburgoWebFinally, the cells were stained with Alexa Phalloidin 555, to bind the actin filaments and highlight the cell morphology. ... (488 nm). The zeta potential of the hybrid Rh-CQDs NPs was estimated as −28 ± 1 mV, revealing the presence of carboxyl groups on the surface of the hybrid NPs, even after the conjugation process. Moreover, the ... almirantazgo de castillaWebAlexa Fluor 488™ phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor™ 488 dye. Also available as a … almirante chesco s.a.sWebMar 30, 2024 · For actin staining, Alexa Fluor 488 phalloidin (Molecular Probes, 1 μL per 22 × 22 mm coverslip) was added to the secondary antibody step. All coverslips were then mounted onto Mowiol supplemented with 0.1 μg/mL DAPI (Sigma-Aldrich). Cells were observed on confocal microscope (Leica TCS SP8) with HC PL APO 63×/1.40 oil … almirante cheryl lyda d md